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BKMS-react is an integrated and non-redundant biochemical reaction database containing known enzyme-catalyzed and spontaneous reactions. Biochemical reactions collected from BRENDA, KEGG, MetaCyc and SABIO-RK were matched and integrated by aligning substrates and products.
 

:= BRENDA, := KEGG, := MetaCyc, := SABIO-RK
:= amino acid sequences := show the reaction diagram
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  • EC Number
  • Reaction
  • Pathways
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  • Stoichiometry Check
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  • Commentary
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EC Number
Reaction
Pathways
Reaction IDs
Stoichiometry Check  
Commentary
isoflavone 7-O-methyltransferase
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone = S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
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: Isoflavone O-methylation is a key reaction for the formation of pterocarpan phytoalexins such as medicarpin. Isoflavone specific O-methyltransferases have been purified expressing a high in vitro affinity for the methylation of the A-ring 7-hydroxyl group of daidzein. The resulting product, isoformononetin (7-O-methyldaidzein), is a rare naturally occurring compound which is unlikely to be involved in the formation of medicarpin. No products of the 7-O-methylation of daidzein have been demonstrated to accumulate in elicited alfalfa cell cultures suggesting that this reaction may not occur in vivo. Recently, a daidzein-7-O-methyltransferase (D7OMT) has been purified from Glycyrrhiza echinata. [12610212] The distinct biochemistry of D7OMT and the distant phylogenetic relationship to another O-methyltransferase (2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase - HI4'OMT) acting specifically on 2,7,4'-trihydroxyisoflavanone implies a new scheme of formonetin biosynthesis which excludes the 4-O-methylation of daidzein.
flavonoid 7-O-methyltransferase
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone = S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
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: Isoflavone O-methylation is a key reaction for the formation of pterocarpan phytoalexins such as medicarpin. Isoflavone specific O-methyltransferases have been purified expressing a high in vitro affinity for the methylation of the A-ring 7-hydroxyl group of daidzein. The resulting product, isoformononetin (7-O-methyldaidzein), is a rare naturally occurring compound which is unlikely to be involved in the formation of medicarpin. No products of the 7-O-methylation of daidzein have been demonstrated to accumulate in elicited alfalfa cell cultures suggesting that this reaction may not occur in vivo. Recently, a daidzein-7-O-methyltransferase (D7OMT) has been purified from Glycyrrhiza echinata. [12610212] The distinct biochemistry of D7OMT and the distant phylogenetic relationship to another O-methyltransferase (2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase - HI4'OMT) acting specifically on 2,7,4'-trihydroxyisoflavanone implies a new scheme of formonetin biosynthesis which excludes the 4-O-methylation of daidzein.